| Column | Ultisil® XB-NH2（4.6×250mm，5μm) |
| Mobile Phase | 0.05mol/L potassium dihydrogen phosphate solution (pH4.0)/acetonitrile =30/70 |
| Detection | 215nm |
| Temperature | 30℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 20μL |
| Note | After normal activation transition of the new column, a small flow rate of mobile phase should be used to balance overnight before normal detection |
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