| Column | Ultisil® MM C18/SCX（4.6×250mm，5μm) |
| Mobile Phase | Mobile phase: 25μL chromatographic grade acetonitrile and 30μL sulfuric acid were added to 1000mL Wahaha water, shaken well, and ultrasonic degassed to obtain |
| Detection | Differential refraction detector 35℃ |
| Temperature | 10℃ |
| Flow Rate | 0.5 ml/min |
| Injection Volume | 10 μL |
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