
Column | Ultisil® MM C18/SCX(4.6×250mm,5μm) |
Mobile Phase | Mobile phase: 25μL chromatographic grade acetonitrile and 30μL sulfuric acid were added to 1000mL Wahaha water, shaken well, and ultrasonic degassed to obtain |
Detection | Differential refraction detector 35℃ |
Temperature | 10℃ |
Flow Rate | 0.5 ml/min |
Injection Volume | 10 μL |
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