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Determination of Glucosamine Hydrochloride and Chondroitin Sulfate by Ultisil AQ-C18

Reference Substance

Column Ultisil® AQ-C18 4.6×250 mm, 5 μm
Mobile Phase Acetonitrile: 10mmol/L Sodium pentanesulfonate solution = 10:90
Detection 192 nm
Temperature 30 ℃
Flow Rate 0.8 ml/min
Injection Volume 10 µl
Note Sensitive to injection volume and dissolving solvent