| Column | Ultisil® ODS-3（4.6×150mm，5μm) |
| Mobile Phase | Mobile phase: Take 300mL tetrahydrofuran, add 700mL Wahaha water, then add 10mL glacial acetic acid, shake well, and degas it by ultrasonic. Water/THF/glacial acetic acid=70/30/1 |
| Detection | 254nm 272nm |
| Temperature | 20℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 20 μL |
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