ColumnUltisil® ODS-3(4.6×150mm,5μm)
Mobile PhaseMobile phase: Take 300mL tetrahydrofuran, add 700mL Wahaha water, then add
10mL glacial acetic acid, shake well, and degas it by ultrasonic.
Water/THF/glacial acetic acid=70/30/1
Detection254nm 272nm
Flow Rate1.0 ml/min
Injection Volume20 μL

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