System suitability solution (254 nm)

ColumnUltisil®ODS-3(
4.6×150mm,5μm)
Mobile PhaseMobile phase: take 300ml of tetrahydrofuran, add 700ml of
water, add 10ml of glacial acetic acid, shake well and degass by ultrasonic.

Water / tetrahydrofuran / glacial acetic acid = 70 / 30 / 1
Detection254nm 272nm
Temperature20℃
Flow Rate 1.0ml/min
Injection Volume20ul
Note/

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