ColumnXtimate® C18(4.6×250mm,5μm)
Mobile PhaseAcetonitrile/triethylamine buffer (take 5ml of triethylamine, add 5ml of
phosphoric acid, add water to 1000ml,
Adjust pH to 2.2 with phosphoric acid) = 65/35
Detection215nm
Temperature30℃
Flow Rate1.0 ml/min
Injection Volume20 μL

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