
Column | Xtimate® C18(4.6×250mm,5μm) |
Mobile Phase | Acetonitrile/triethylamine buffer (take 5ml of triethylamine, add 5ml of phosphoric acid, add water to 1000ml, Adjust pH to 2.2 with phosphoric acid) = 65/35 |
Detection | 215nm |
Temperature | 30℃ |
Flow Rate | 1.0 ml/min |
Injection Volume | 20 μL |
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