ColumnUltisil® UHPLC Polar RP(2.1×100mm,1.8μm)
Mobile PhaseMobile phase: take 11.16g potassium dihydrogen phosphate and 7.2g dipotassium
hydrogen phosphate trihydrate, add 1000mL Wahaha water, mix and suction filter.
Take 880mL of the above solution and 120mL chromatographic grade methanol,
and then adjust the pH to 1mol/L phosphoric acid. 3.0, ultrasonic degassing, that
is.
Phosphate buffer/methanol = 88/12
Detection280nm
Temperature35℃
Flow Rate0.15 ml/min
Injection Volume2 μL

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