| Column | Ultisil® UHPLC Polar RP（2.1×100mm，1.8μm) |
| Mobile Phase | Mobile phase: take 11.16g potassium dihydrogen phosphate and 7.2g dipotassium hydrogen phosphate trihydrate, add 1000mL Wahaha water, mix and suction filter. Take 880mL of the above solution and 120mL chromatographic grade methanol, and then adjust the pH to 1mol/L phosphoric acid. 3.0, ultrasonic degassing, that is. Phosphate buffer/methanol = 88/12 |
| Detection | 280nm |
| Temperature | 35℃ |
| Flow Rate | 0.15 ml/min |
| Injection Volume | 2 μL |
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