
Column | Xtimate® SEC-120 (7.8×300 mm, 3 μm) (two in series) |
Mobile Phase | A (phosphate buffer (0.005 mol/L disodium hydrogen phosphate: 0.005 mol/L sodium dihydrogen phosphate = 61:39, pH=7.0)): B (acetonitrile) = 95:5 |
Detection | 220nm |
Temperature | 40℃ |
Flow Rate | 0.6 ml/min |
Injection Volume | 20 μL |
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