ColumnXtimate® SEC-120 (7.8×300 mm, 3 μm) (two in series)
Mobile PhaseA (phosphate buffer (0.005 mol/L disodium hydrogen phosphate: 0.005 mol/L
sodium dihydrogen phosphate = 61:39, pH=7.0)): B (acetonitrile) = 95:5
Detection220nm
Temperature40℃
Flow Rate0.6 ml/min
Injection Volume20 μL

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