Reference

ColumnWelch Ultisil XB-C18 4.6×150mm,3μm
Mobile PhaseWater: acetonitrile = 72: 28 (0 min, 9 min), 28: 72 (39 min), 72: 28 (42 min, 52 min);
Detector232 nm
Temperature30℃
Flow Rate1.2ml/min
Injection Volume10ul

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