Column temperature 45℃

ColumnUltisil® Polar-RP(4.6×150mm,3.5μm)
Detection232nm
Temperature45℃
Flow Rate1.2ml/min
Injection Volume10ul
Note/

Mobile Phase

Time(min)Purified water(%)Acetonitrile (%)
07228
9.07228
39.02872
49.00100
49.17228
607228

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