ColumnUltisil® XB-C18 (4.6×150mm,3μm)
Mobile Phas5mmol/L ammonium acetate solution (containing 1% triethylamine, adjust pH=5.0
with glacial acetic acid)/acetonitrile=35/65
Detection214nm
Temperature30℃
Flow Rate1.0 ml/min
Injection Volume20 μL

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