| Column | Ultisil® ODS-3（4.6×250mm，5μm) |
| Mobile Phase | Weigh 3.11g of dioctyl sodium sulfosuccinate, add 400ml and 600ml of water to dissolve, add nitrate Sodium 0.56g, adjust pH to 2.0 with glacial acetic acid |
| Detection | 280nm |
| Temperature | 25℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 20 μL |
| Note | The column needs to be equilibrated overnight with a low flow of mobile phase |
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