Reference solution
Column | Ultisil Polar RP 4.6×250,5μm |
Mobile Phase | Water-acetonitrile-methanol(75:5:20) |
Detector | 269nm |
Temperature | 35℃ |
Flow Rate | 1.0ml/min |
Injection Volume | 20ul |
Notes | Pressure is relatively small at 35℃ than that of room temperature |
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