Method 1:

ColumnUltisil® Cellu-DR(4.6×150mm,5μm)
Mobile PhaseMobile phase A: methanol/ethanol/diethylamine=90/10/0.1
Mobile phase A/water=95/5
Detection220nm
Temperature30℃
Flow Rate0.6 ml/min
Injection Volume2 μL

Method 2:

ColumnUltisil® Cellu-JR(4.6×150mm,5μm)
Mobile PhaseMethanol/Ethanol/Diethylamine=90/10/0.1
Detection220nm
Temperature30℃
Flow Rate0.8 ml/min
Injection Volume5 μL

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