| Column | Ultisil® AQ C18（4.6×150mm，5μm) |
| Mobile Phase | Ammonium acetate buffer salt: Take 4.0g ammonium acetate and 7.0g sodium perchlorate monohydrate, add 1300mL Wahaha water, then adjust the pH to 2.2 with phosphoric acid, shake well, ultrasonic degassification, and get. Ammonium acetate buffer/acetonitrile =83/17 |
| Detection | 332nm |
| Temperature | 40℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 5 μL |
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