ColumnUltisil® AQ C18(4.6×150mm,5μm)
Mobile PhaseAmmonium acetate buffer salt: Take 4.0g ammonium acetate and 7.0g sodium
perchlorate monohydrate, add 1300mL Wahaha water, then adjust the pH to 2.2
with phosphoric acid, shake well, ultrasonic degassification, and get.
Ammonium acetate buffer/acetonitrile =83/17
Detection332nm
Temperature40℃
Flow Rate1.0 ml/min
Injection Volume5 μL

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