| Column | Welch Ultisil® LP-C18（4.6×250mm，5μm） |
| Mobile Phase | Mobile Phase A: Take 100 mL of chromatography-grade acetonitrile and mix with 900 mL of Wahaha water, shake well, and sonicate to remove air bubbles. Mobile Phase B: Take 600 mL of chromatography-grade acetonitrile and mix with 400 mL of Wahaha water, shake well, and sonicate to remove air bubbles. Time (min) Mobile Phase A Mobile Phase B 0 60 40 5 60 40 15 0 100 20 0 100 20.1 60 40 30 60 40 |
| Column Temperature | 30 ℃ |
| Detection wavelength: | 230nm |
| Flow Rate | 1.5 ml/min |
| Injection Volume | 100 μL |
| Note | / |
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