| Column | Xtimate® C18（4.6×250mm，5μm) |
| Mobile Phase | See the table below for details |
| Detection | ELSD 60 ℃ |
| Temperature | 30℃ |
| Flow Rate | 0.6 ml/min |
| Injection Volume | 10 μL |
Mobile Phase:
| Time (min) | Mobile phase A(%) | Mobile phase B(%) |
| 0 | 20 | 80 |
| 10 | 10 | 90 |
| 35 | 10 | 90 |
| 35.1 | 20 | 80 |
| 40 | 20 | 80 |
If you have any problem or require further information, please contact info@welchmat.com.
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