ColumnWelch Ultisil XB-C18 4.6×250mm,5μm
Mobile PhaseA:Acetonitrile-0.1mol / L sodium acetate solution (adjust pH to 6.5 with acetic acid) = 7:93 B:Acetonitrile: water = 4:1
Detector254nm
Temperature43℃
Flow Rate1ml/min
Injection Volume5ul

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