System suitability solution(Full Scale)

ColumnXtimate® C18, 4.6×250mm, 5μm
Detection257 nm
Temperature20℃
Flow Rate1.2 mL/min
Injection Volume20 µL
NotesA Ghost-buster column should be installed before injector to remove ghost peaks

Mobile Phase

Time(min)Phosphate buffer (%)Acetonitrile/methanol =55/45 (%)
08020
202080
301090
318020
458020

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