System Suitability

ColumnUltisil XB-C18, 5μm, 4.6×150mm
Mobile PhaseMethanol: Buffer(adjust pH to 5.7 with 10% phosphate) =70:30
Detection215nm
Temperature30℃
Flow Rate1.0ml/min
Injection Volume10ul
NoteAdjust mobile phase pH only after methanol and buffer well mixed

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