Determination of Cis-trans Isomers in Vitamin K1

Vitamin K1, also known as the plant methylnaphthoquinone, chlorphenoquinone, chlorphenoquinone, 2-methyl-3- phytol-1,4-naphthoquinone, is a polycyclic aromatic ketone and fat-soluble vitamin. Extensively existing in natural green plants, Vitamin K1 is stable in the air and moisture, but it will be decomposed in the sun.

As the necessary substance for liver synthesis factors II, VII, IX, X,  Vitamin K1 is mainly used for the treatment of hemorrhagic diseases caused by vitamin K deficiency.

Constitutional Formula

Chromatographic Condition

Column: Topsil^®Silica（4.6×300mm，5μm)

Mobile phase: Petroleum ether (60~90℃)/n-pentanol =2000/2.5;

Wavelength: 254nm

Column Temp：40℃

Flow rate：0.6ml/min

Injection volume：10μL

Reference Substance Solution

Peak	Rt	Area	Height	Plates	Resolution	Tailing factor
1	13.274	3353330	276291	26482	0.00	1.011
2	14.613	22465941	1328178	17015	3.46	1.417
Total	_	25819271	1604469	_	_	_

Test Sample Solution

Peak	Rt	Area	Height	Plates	Resolution	Tailing factor
1	13.274	6692432	536722	25421	0.00	1.049
2	14.619	21679771	1295948	17458	3.54	1.387
Total	_	28372203	1832670	_	_	_

Conclusion

Using Topsil^® Silica（4.6×300mm，5 μm)  under this chromatographic condition can meet the test requirements.

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