Reference solution

ColumnUltisil® XB-C18(4.6×300mm,5μm)
Detection262nm/272nm
Temperature30℃
Flow Rate1.5ml/min
Injection Volume20ul
Note/

Mobile Phase
Mobile phase A: take 1.66mL phosphoric acid, add 1000mL Wahaha water, take 870mL phosphorus The acid solution was mixed with 130 mL of acetonitrile, adjusted to pH 3.0 ± 0.1 with triethylamine, filtered with suction, and degassed by ultrasonic.

Time(min)Mobile phase A (%)Acetonitrile (%)
01000
161000
534060
541000
651000

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