Reference solution

Column | Ultisil® XB-C18(4.6×300mm,5μm) |
Detection | 262nm/272nm |
Temperature | 30℃ |
Flow Rate | 1.5ml/min |
Injection Volume | 20ul |
Note | / |
Mobile Phase
Mobile phase A: take 1.66mL phosphoric acid, add 1000mL Wahaha water, take 870mL phosphorus The acid solution was mixed with 130 mL of acetonitrile, adjusted to pH 3.0 ± 0.1 with triethylamine, filtered with suction, and degassed by ultrasonic.
Time(min) | Mobile phase A (%) | Acetonitrile (%) |
0 | 100 | 0 |
16 | 100 | 0 |
53 | 40 | 60 |
54 | 100 | 0 |
65 | 100 | 0 |
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