Reference solution
| Column | Ultisil® XB-C18（4.6×300mm，5μm) |
| Detection | 262nm/272nm |
| Temperature | 30℃ |
| Flow Rate | 1.5ml/min |
| Injection Volume | 20ul |
| Note | / |
Mobile Phase
Mobile phase A: take 1.66mL phosphoric acid, add 1000mL Wahaha water, take 870mL phosphorus The acid solution was mixed with 130 mL of acetonitrile, adjusted to pH 3.0 ± 0.1 with triethylamine, filtered with suction, and degassed by ultrasonic.
| Time(min） | Mobile phase A (%) | Acetonitrile (%) |
| 0 | 100 | 0 |
| 16 | 100 | 0 |
| 53 | 40 | 60 |
| 54 | 100 | 0 |
| 65 | 100 | 0 |
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