Reference solution

ColumnUltisil® XB-C18(4.6×250mm,5μm)
Detection348nm
Temperature35℃
Flow Rate1.0ml/min
Injection Volume5ul
NoteAfter 30-40 minutes of gradient, the proportion of organic phase in
the elution section is adjusted, which meets the requirements of
Pharmacopoeia. If the impurities cannot be eluted according to the
original proportion, they will remain in the next needle and interfere
with the detection of the next needle

Mobile Phase

Time(min)0.1% phosphoric acid (%)acetonitrile (%)
09010
118218
308020
404060

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