Reference solution
| Column | Ultisil® XB-C18（4.6×250mm，5μm) |
| Detection | 348nm |
| Temperature | 35℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 5ul |
| Note | After 30-40 minutes of gradient, the proportion of organic phase in the elution section is adjusted, which meets the requirements of Pharmacopoeia. If the impurities cannot be eluted according to the original proportion, they will remain in the next needle and interfere with the detection of the next needle |
Mobile Phase
| Time(min） | 0.1% phosphoric acid (%) | acetonitrile (%) |
| 0 | 90 | 10 |
| 11 | 82 | 18 |
| 30 | 80 | 20 |
| 40 | 40 | 60 |
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