Sample Diagram
| Column | Ultisil C18（4.6*250mm，5μm） |
| Mobile Phase | A: Phosphate buffer, B: Acetonitrile |
| Detection | 225nm |
| Temperature | 30℃ |
| Flow rate | 1.2ml/min |
| Injection Volume | 10ul |
| Note | Sensitive to injection volume and dissolved sample solvent |
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