Related substance system suitability
Column | Ultisil® LP-C8, 5μm, 4.6×250mm PN: 00209-31043 |
Detection | 273 nm |
Temperature | 30℃ |
Flow Rate | 1.5 ml/min |
Injection Volume | 20 µl |
Mobile Phase
Time (min) | pH3.4 acetate buffer (%) | Acetonitrile (%) |
0 | 95 | 5 |
40 | 80 | 20 |
50 | 60 | 40 |
51 | 95 | 5 |
55 | 95 | 5 |
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