Blank solution
| Column | Xtimate® C18（ 4.6×200mm，5μm) |
| Detection | 254nm |
| Temperature | 30℃ |
| Flow Rate | 0.8ml/min |
| Injection Volume | 20ul |
| Note | / |
Mobile Phase
Mobile phase A; 281.1000mg of Butyl Ammonium Mobile phase A: weigh 1.42g citric acid, 2.31g disodium hydrogen phosphate, and dissolve and dilute with water, 1000ml (if necessary, adjust the pH value to 3.6 with phosphoric acid or sodium hydroxide solution), mix and filter,Readily available;
| Time(min） | Mobile phase A (%) | Acetonitrile (%) |
| 0 | 97 | 3 |
| 10 | 85 | 15 |
| 20 | 40 | 60 |
| 25 | 40 | 60 |
| 26 | 97 | 3 |
| 35 | 97 | 3 |
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