| Column | Ultisil® Plus C18（4.6×150mm，5μm) |
| Mobile Phase | See the table below for details |
| Detection | 255nm |
| Temperature | 35℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 20 μL |
Mobile Phase:
Mobile Phase A: Acetonitrile
Mobile Phase B: Phosphate Buffer (pH=3.9)
| Time (min) | Mobile phase A(%) | Mobile phase B(%) |
| 0 | 7 | 93 |
| 14 | 7 | 93 |
| 29 | 14 | 86 |
| 40 | 14 | 86 |
| 41 | 7 | 93 |
| 52 | 7 | 93 |
If you have any problem or require further information, please contact info@welchmat.com.
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