ColumnUltisil® Polar-RP(4.6×250mm,5μm)
Mobile PhaseMobile phase A: Phosphate buffer (dissolve 7.1g of anhydrous disodium hydrogen
phosphate in 1000ml of water, adjust pH to 6.25 with phosphoric
Detection254nm
Temperature30℃
Flow Rate1.0 ml/min
Injection Volume20 μL

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