| Column | Ultisil® Polar-RP（4.6×250mm，5μm) |
| Mobile Phase | Mobile phase A: Phosphate buffer (dissolve 7.1g of anhydrous disodium hydrogen phosphate in 1000ml of water, adjust pH to 6.25 with phosphoric |
| Detection | 254nm |
| Temperature | 30℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 20 μL |
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