ColumnUltisil® Polar-RP(4.6×250mm,5μm)
Xtimate®C18(4.6×250mm,5μm)
Mobile PhaseAcetonitrile-pH7.0 Phosphate Buffer-Water (40:200:1760)
Detection254nm
Temperature30℃
Flow Rate1.5 ml/min
Injection Volume20 μL

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