Resolution solution
| Column | Ultisil®Alk C18（ 4.6×200mm，5μm) |
| Mobile Phase | Phosphate buffer: Dissolve 5.0 g of ammonium dihydrogen phosphate in 750 mL of Wahaha water, adjust the pH to 3.5 with phosphoric acid, add 120 mL of Wahaha water, mix well, and ultrasonically degas. Phosphate buffer/acetonitrile = 870/130 |
| Detection | 254nm |
| Temperature | 25℃ |
| Flow Rate | 2.3ml/min |
| Injection Volume | 5ul |
| Note | / |
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