PBS blank solution
| Column | Ultisil® Plus-Phenyl（4.6×250mm，5μm) |
| Detection | 235nm |
| Temperature | 25℃ |
| Flow Rate | 0.9ml/min |
| Injection Volume | 20ul |
| Note | / |
Mobile Phase
A: Buffer salt solution B: methanol
| Time(min） | Mobile phase A (%) | Mobile phase B (%) |
| 0 | 90 | 10 |
| 12 | 90 | 10 |
| 37 | 80 | 20 |
| 50 | 60 | 40 |
| 55 | 20 | 80 |
| 60 | 20 | 80 |
If you have any problem or require further information, please contact info@welchmat.com.
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