| Column | Ultisil® Plus C18（4.6×150mm，5μm) |
| Mobile Phase | Phosphate buffer (take 7.1g of anhydrous disodium hydrogen phosphate, dissolve it in 1000ml of water, adjust pH6.25 with phosphoric acid)/methanol=85/15 |
| Detection | 235nm |
| Temperature | 30℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 20 μL |
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