| Column | Ultisil® Alk C18（4.6×200mm，5μm) |
| Mobile Phase | Phosphate buffer: Take 5.0g ammonium dihydrogen phosphate, add 750mL Wahaha water to dissolve, adjust pH to 3.5 with phosphoic acid, then add 120mL Wahaha water to mix, and ultrasonic degassed, to obtain. Phosphate buffer/acetonitrile =870/130 |
| Detection | 254nm |
| Temperature | 25℃ |
| Flow Rate | 2.3 ml/min |
| Injection Volume | 5 μL |
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