ColumnUltisil® Alk C18(4.6×200mm,5μm)
Mobile PhasePhosphate buffer: Take 5.0g ammonium dihydrogen phosphate, add 750mL
Wahaha water to dissolve, adjust pH to 3.5 with phosphoic acid, then add 120mL
Wahaha water to mix, and ultrasonic degassed, to obtain.
Phosphate buffer/acetonitrile =870/130
Detection254nm
Temperature25℃
Flow Rate2.3 ml/min
Injection Volume5 μL

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