| Column | Ultisil®XB-C30(4.6×250 mm,3 μm) |
| Mobile Phase | See the table below for details |
| Temperature | 30℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 20 μL |
| Note | The sample is unstable, it is recommended to operate in a low temperature and dark environment |
Mobile Phase：(A: methanol/acetonitrile/water = (73.5/24.5/2) B: methyl tert-butyl ether)
| Time (min) | Mobile phase A(%) | Mobile phase B(%) |
| 0 | 100 | 0 |
| 15 | 59 | 41 |
| 18 | 20 | 80 |
| 19 | 20 | 80 |
| 20 | 0 | 100 |
| 22 | 100 | 0 |
| 32 | 100 | 0 |
If you have any problem or require further information, please contact info@welchmat.com.
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