Application range
This method is applicable to detection of cannabinol content in skincare oil by HPLC.
Purification steps
SPE column: Welchrom® Alumina-N, 1000mg/6mL
Activation: 10mL ethyl acetate, 5mL acetonitrile, discard
Sample loading: load all samples, then collect
Elution: 15mL ethyl acetate, combine and collect
Concentration: blow with nitrogen to dry at room temperature, then add 1mL acetonitrile to redissolve. Filter through 0.22 μm NY filter membrane for detection
Chromatographic conditions
Column: Ultisil® XB-C18, 4.6*150mm, 5μm
Mobile phase: 0.015mol/L Sodium dihydrogen phosphate buffer (pH 4.0) -acetonitrile =25: 75
Flow rate: 1.0mL/min
Column temperature: 30 ℃
Injection volume: 10 μL.
Chromatograpm or spike recovery rate
Fig 1. Chromatograpm of cannabinol 1.0mg/L
Fig 2. Chromatograpm of blank sample of skin care oil
Fig 3. Chromatograpm of skin oil sample at 3.33mg/kg
Tab. Spike recovery rate
| Substance | Spike Level | Average Recovery Rate | RSD (n=2) |
| Skin care oil | 3.33mg/kg | 83 | 2.67 |
Ordering information
| P/N | Product | Specification |
| 00518-20007 | SPE column | Welchrom® Alumina-N, 1000mg/6mL, 30pk |
| 00837-05006 | 50mL centrifuge tube with screw mouth and apical bottom | Disposable centrifuge tube, flat cap, conical bottom, RCF12000xg, 50 mL, 50/ pk without sterilization |
| 00837-05002 | 15mL centrifuge tube with screw mouth and conical bottom | Disposable centrifuge tube, flat cap, conical bottom, RCF12000xg, 15 mL, 50/ pk without sterilization |
| 00824-31001 | Welch SPE instrument | 12 ports square cylinder |
| 00821-32291 | Caps and septa | Pre-slit red PTFE/white Silicone septa, 9mm blue short screw-thread polypropylene cap, 6mm centre hole, 100/pk |
If you have any problem or require further information, please contact info@welchmat.com.
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