Determination of Cannabidiol Content in Skincare Oil

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Application range

This method is applicable to detection of cannabinol content in skincare oil by HPLC.

Purification steps

SPE column: Welchrom® Alumina-N, 1000mg/6mL

Activation: 10mL ethyl acetate, 5mL acetonitrile, discard

Sample loading: load all samples, then collect

Elution: 15mL ethyl acetate, combine and collect

Concentration: blow with nitrogen to dry at room temperature, then add 1mL acetonitrile to redissolve. Filter through 0.22 μm NY filter membrane for detection

Chromatographic conditions

Column: Ultisil® XB-C18, 4.6*150mm, 5μm

Mobile phase: 0.015mol/L Sodium dihydrogen phosphate buffer (pH 4.0) -acetonitrile =25: 75

Flow rate: 1.0mL/min

Column temperature: 30 ℃

Injection volume: 10 μL.

Chromatograpm or spike recovery rate

Fig 1. Chromatograpm of cannabinol 1.0mg/L

Fig 2. Chromatograpm of blank sample of skin care oil

Fig 3. Chromatograpm of skin oil sample at 3.33mg/kg

Tab. Spike recovery rate

SubstanceSpike LevelAverage Recovery RateRSD (n=2)
Skin care oil3.33mg/kg832.67

Ordering information

P/NProductSpecification
00518-20007SPE columnWelchrom® Alumina-N, 1000mg/6mL, 30pk
00837-0500650mL centrifuge tube with screw mouth and apical bottomDisposable centrifuge tube, flat cap, conical bottom, RCF12000xg, 50 mL, 50/ pk without sterilization
00837-0500215mL centrifuge tube with screw mouth and conical bottomDisposable centrifuge tube, flat cap, conical bottom, RCF12000xg, 15 mL, 50/ pk without sterilization
00824-31001Welch SPE instrument12 ports square cylinder
00821-32291Caps and septaPre-slit red PTFE/white Silicone septa, 9mm blue short screw-thread polypropylene cap, 6mm centre hole, 100/pk

If you have any problem or require further information, please contact info@welchmat.com.


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