
Application range
This method is applicable to detection of cannabinol content in skincare oil by HPLC.
Purification steps
SPE column: Welchrom® Alumina-N, 1000mg/6mL
Activation: 10mL ethyl acetate, 5mL acetonitrile, discard
Sample loading: load all samples, then collect
Elution: 15mL ethyl acetate, combine and collect
Concentration: blow with nitrogen to dry at room temperature, then add 1mL acetonitrile to redissolve. Filter through 0.22 μm NY filter membrane for detection
Chromatographic conditions
Column: Ultisil® XB-C18, 4.6*150mm, 5μm
Mobile phase: 0.015mol/L Sodium dihydrogen phosphate buffer (pH 4.0) -acetonitrile =25: 75
Flow rate: 1.0mL/min
Column temperature: 30 ℃
Injection volume: 10 μL.
Chromatograpm or spike recovery rate

Fig 1. Chromatograpm of cannabinol 1.0mg/L

Fig 2. Chromatograpm of blank sample of skin care oil

Fig 3. Chromatograpm of skin oil sample at 3.33mg/kg
Tab. Spike recovery rate
Substance | Spike Level | Average Recovery Rate | RSD (n=2) |
Skin care oil | 3.33mg/kg | 83 | 2.67 |
Ordering information
P/N | Product | Specification |
00518-20007 | SPE column | Welchrom® Alumina-N, 1000mg/6mL, 30pk |
00837-05006 | 50mL centrifuge tube with screw mouth and apical bottom | Disposable centrifuge tube, flat cap, conical bottom, RCF12000xg, 50 mL, 50/ pk without sterilization |
00837-05002 | 15mL centrifuge tube with screw mouth and conical bottom | Disposable centrifuge tube, flat cap, conical bottom, RCF12000xg, 15 mL, 50/ pk without sterilization |
00824-31001 | Welch SPE instrument | 12 ports square cylinder |
00821-32291 | Caps and septa | Pre-slit red PTFE/white Silicone septa, 9mm blue short screw-thread polypropylene cap, 6mm centre hole, 100/pk |
If you have any problem or require further information, please contact info@welchmat.com.
2 Comments
Форекс брокерийн компани · July 8, 2021 at 11:29 pm
It’s an amazing piece of writing for all the internet visitors;
they will take benefit from it I am sure.
THC · June 30, 2021 at 2:39 am
you’re actually a just right webmaster. The web site loading velocity is amazing.
It kind of feels that you’re doing any distinctive trick.
Furthermore, The contents are masterwork. you have performed a excellent
task on this topic!