Determination of Cannabidiol Content in Skincare Oil

Published by News Editor on

Application range

This method is applicable to detection of cannabinol content in skincare oil by HPLC.

Purification steps

SPE column: Welchrom® Alumina-N, 1000mg/6mL

Activation: 10mL ethyl acetate, 5mL acetonitrile, discard

Sample loading: load all samples, then collect

Elution: 15mL ethyl acetate, combine and collect

Concentration: blow with nitrogen to dry at room temperature, then add 1mL acetonitrile to redissolve. Filter through 0.22 μm NY filter membrane for detection

Chromatographic conditions

Column: Ultisil® XB-C18, 4.6*150mm, 5μm

Mobile phase: 0.015mol/L Sodium dihydrogen phosphate buffer (pH 4.0) -acetonitrile =25: 75

Flow rate: 1.0mL/min

Column temperature: 30 ℃

Injection volume: 10 μL.

Chromatograpm or spike recovery rate

Fig 1. Chromatograpm of cannabinol 1.0mg/L

Fig 2. Chromatograpm of blank sample of skin care oil

Fig 3. Chromatograpm of skin oil sample at 3.33mg/kg

Tab. Spike recovery rate

SubstanceSpike LevelAverage Recovery RateRSD (n=2)
Skin care oil3.33mg/kg832.67

Ordering information

00518-20007SPE columnWelchrom® Alumina-N, 1000mg/6mL, 30pk
00837-0500650mL centrifuge tube with screw mouth and apical bottomDisposable centrifuge tube, flat cap, conical bottom, RCF12000xg, 50 mL, 50/ pk without sterilization
00837-0500215mL centrifuge tube with screw mouth and conical bottomDisposable centrifuge tube, flat cap, conical bottom, RCF12000xg, 15 mL, 50/ pk without sterilization
00824-31001Welch SPE instrument12 ports square cylinder
00821-32291Caps and septaPre-slit red PTFE/white Silicone septa, 9mm blue short screw-thread polypropylene cap, 6mm centre hole, 100/pk

If you have any problem or require further information, please contact


Форекс брокерийн компани · July 8, 2021 at 11:29 pm

It’s an amazing piece of writing for all the internet visitors;
they will take benefit from it I am sure.

THC · June 30, 2021 at 2:39 am

you’re actually a just right webmaster. The web site loading velocity is amazing.

It kind of feels that you’re doing any distinctive trick.
Furthermore, The contents are masterwork. you have performed a excellent
task on this topic!

Leave a Reply

Avatar placeholder

Your email address will not be published. Required fields are marked *