Solution of reference

ColumnUltisil Plus C18 4.6×250mm, 5μm
  Mobile PhaseA:Acetonitrile: Glacial acetic acid: Water = 57:1:43 B:Acetonitrile: Glacial acetic acid: Water = 90:1:10
Detector254nm
TemperatureRoom Temperature
Flow Rate1 ml/min
Injection Volume10µl

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