Sample Solution 2 (0.4mg/ml solid 1 and 0.8ug/ml solid 2 mixed solution):

ColumnUltisil® LP-C8, 5μm, 4.6×250mm    PN: 00209-31043
Detection285 nm
Temperature40℃
Flow Rate1 ml/min
Injection Volume20 µl

Mobile Phase

A: 1mL TFA add into 1000mL water B: Acetonitrile

Time (min)A%B%
08515
38515
30595
33595

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