
Column | Ultisil® XS-C18(4.6×250mm,5μm) |
Mobile Phase | See the table below for details |
Detection | 240nm |
Temperature | 45℃ |
Flow Rate | 1.0 ml/min |
Injection Volume | 50 μL |
Note | If there are ghost peaks in the blank, add an impurity trap column to remove them |
Mobile Phase:
Mobile phase A: 0.05% glacial acetic acid in water (v/v) (adjust pH to 3.9 with potassium hydroxide solution) Mobile Phase B: Acetonitrile
Time (min) | Mobile phase A(%) | Mobile phase B(%) |
0 | 75 | 25 |
5 | 75 | 25 |
35 | 68 | 32 |
42 | 59 | 41 |
56 | 30 | 70 |
65 | 25 | 75 |
70 | 25 | 75 |
75 | 75 | 25 |
88 | 75 | 25 |
If you have any problem or require further information, please contact info@welchmat.com.
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