| Column | Ultisil® XS-C18（4.6×250mm，5μm) |
| Mobile Phase | See the table below for details |
| Detection | 240nm |
| Temperature | 45℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 50 μL |
| Note | If there are ghost peaks in the blank, add an impurity trap column to remove them |
Mobile Phase：
Mobile phase A: 0.05% glacial acetic acid in water (v/v) (adjust pH to 3.9 with potassium hydroxide solution) Mobile Phase B: Acetonitrile
| Time (min) | Mobile phase A(%) | Mobile phase B(%) |
| 0 | 75 | 25 |
| 5 | 75 | 25 |
| 35 | 68 | 32 |
| 42 | 59 | 41 |
| 56 | 30 | 70 |
| 65 | 25 | 75 |
| 70 | 25 | 75 |
| 75 | 75 | 25 |
| 88 | 75 | 25 |
If you have any problem or require further information, please contact info@welchmat.com.
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