ColumnUltisil® XS-C18(4.6×250mm,5μm)
Mobile PhaseSee the table below for details
Detection240nm
Temperature45℃
Flow Rate1.0 ml/min
Injection Volume50 μL
NoteIf there are ghost peaks in the blank, add an impurity trap column to remove them

Mobile Phase

Mobile phase A: 0.05% glacial acetic acid in water (v/v) (adjust pH to 3.9 with potassium hydroxide solution) Mobile Phase B: Acetonitrile

Time (min)Mobile phase A(%)Mobile phase B(%)
07525
57525
356832
425941
563070
652575
702575
757525
887525

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