
Column | Ultisil® Cellu-JR(4.6×250mm,5μm) |
Mobile Phase | See the table below for details |
Detection | 240nm |
Temperature | 30℃ |
Flow Rate | 0.5ml/min |
Injection Volume | 50 μL |
Mobile Phase:(Mobile phase A: 0.05% glacial acetic acid in water (v/v) (adjust pH to 3.9 with potassium hydroxide solution) Mobile Phase B: Acetonitrile)
Time (min) | Mobile phase A(%) | Mobile phase B(%) |
0 | 75 | 25 |
5 | 75 | 25 |
35 | 68 | 32 |
42 | 59 | 41 |
59 | 30 | 70 |
65 | 25 | 75 |
70 | 25 | 75 |
75 | 75 | 25 |
88 | 75 | 25 |
If you have any problem or require further information, please contact info@welchmat.com.
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