Mixed solution of API and impurities – enlarged view
| Column | Ultisil®XB-C18（ 4.6×250mm，5μm) |
| Mobile Phase | 0.01mol/L Potassium dihydrogen phosphate aqueous solution +0.5g/L sodium dodecyl sulfate (pH adjusted to 2.5 with phosphoric acid)/acetonitrile =80 |
| Detection | 205nm |
| Temperature | 30℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 10ul |
| Note | Low flow rate overnight balance helps to stabilize the system |
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