ColumnUltisil® XB-C18(4.6×250mm,5μm)
Mobile Phase0.01mol/L potassium dihydrogen phosphate aqueous solution+0.5g/L sodium
dodecyl sulfate (adjust pH to 2.5 with phosphoric acid)/acetonitrile=80/20
Detection205nm
Temperature30℃
Flow Rate1.0 ml/min
Injection Volume10 μL
NoteOvernight equilibration at a low flow rate is beneficial for stabilizing the system

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