| Column | Ultisil® XB-C18（4.6×250mm，5μm) |
| Mobile Phase | 0.01mol/L potassium dihydrogen phosphate aqueous solution+0.5g/L sodium dodecyl sulfate (adjust pH to 2.5 with phosphoric acid)/acetonitrile=80/20 |
| Detection | 205nm |
| Temperature | 30℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 10 μL |
| Note | Overnight equilibration at a low flow rate is beneficial for stabilizing the system |
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