ColumnColumn 1: Xtimate ® SEC-100, 7.8×300mm, 5μm
Column 2: Xtimate ® SEC-100, 7.8×300mm, 5μm, Xtimate ® SEC-120,
7.8×300mm, 5μm (two in series)
Mobile Phase10mM disodium hydrogen phosphate-10mM sodium dihydrogen phosphate=61-39
Detection294nm
Temperature25℃
Flow Rate0.5 ml/min
Injection Volume10 μL

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