| Column | Column 1: Xtimate ® SEC-100, 7.8×300mm, 5μm Column 2: Xtimate ® SEC-100, 7.8×300mm, 5μm, Xtimate ® SEC-120, 7.8×300mm, 5μm (two in series) |
| Mobile Phase | 10mM disodium hydrogen phosphate-10mM sodium dihydrogen phosphate=61-39 |
| Detection | 294nm |
| Temperature | 25℃ |
| Flow Rate | 0.5 ml/min |
| Injection Volume | 10 μL |
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