Lycium barbarum, also known as wolfberry, red eardrop, is the mature fruit of lycium barbarum, a small shrub of nightshade family. Wolfberry has a long history of homology of medicine and food, is a famous and precious Chinese herbal medicine. Wolfberry contains a variety of amino acids, and contains betaine, zeaxanthin, acid berry red and other special nutrients, so that it has a very good health care effect.

Lycium barbarum is of great significance to human health, can be health care for the body, is people’s favorite fruit. Goji berries are for everyone. Over-use eyes and the elderly are more suitable. Wolfberry nature is relatively mild, eating a little more harmless, but if uncontrolled, eating too much will also make people angry. Dry is easy to preserve, ordinary bags are generally 70% to 80% dry, it is not suitable to accumulate a large number of inventory, often take out under the sun to dry, otherwise it will be sticky and metamorphic for a long time.

In this article, inverse C18 column was used to determine the content of betaine in Wolfberry fruit. Compared with amino column and ion exchange column in many current papers, C18 column has better life and reproducibility.

The chromatographic conditions

Column: Welch Ultisil®LP-C18 (4.6×300mm, 5μm).
Mobile phase: AB two-phase gradient elution;
Detector: Evaporated light scattering detector Drift tube temperature 115℃ carrier gas flow rate 3.5L/min.

Spectrograms and data

Reference solution

Sample Diagram


Use Welch Ultisil® LP-C18 (4.6×300mm, 5μm) to meet the detection requirements under this chromatographic condition.

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