Sample

ColumnXtimate C18 3.0x150mm,3μm 
Mobile PhaseMixture of 350ml acetonitrile and 650ml ammonium acetate buffer (dissolve 0.69g ammonium acetate in 1000ml water, adjust PH to 4.7 with glacial acetic acid), then add 2.88g sodium lauryl sulfate, filter and degass
Detector254nm
Temperature40℃
Flow Rate0.5ml/min
Injection Volume10ul

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