Sample
| Column | Xtimate C18 3.0x150mm,3μm |
| Mobile Phase | Mixture of 350ml acetonitrile and 650ml ammonium acetate buffer (dissolve 0.69g ammonium acetate in 1000ml water, adjust PH to 4.7 with glacial acetic acid), then add 2.88g sodium lauryl sulfate, filter and degass |
| Detector | 254nm |
| Temperature | 40℃ |
| Flow Rate | 0.5ml/min |
| Injection Volume | 10ul |
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