Determination of Berberine Hydrochloride by Ultisil XB-C18

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1. Reference substance

ColumnUltisil XB-C18(4.6×250mm,5μm)
Mobile PhaseAcetonitrile /0.033mol/L potassium dihydrogen phosphate solution =40/60
Detection265nm
Temperature35℃
Flow Rate1.0ml/min
Injection Volume10ul
Note\

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