ColumnUltisil® ODS-3(4.6×250mm,5μm)
Mobile PhaseSee the table below for details
(0.01mol/L ammonium formate: Take 0.63g ammonium formate, add 1000mL
Wahaha water to dissolve, adjust the pH to 3.0 with formic acid, shake well, and
ultrasonic degassification to obtain.
Acetonitrile: chromatographic grade acetonitrile is obtained.)
Detection254nm
Temperature35℃
Flow Rate1.0 ml/min
Injection Volume10 μL

Mobile Phase:

Time (min)0.01 mol/L ammonium formateAcetonitrile
0964
8964
189010
239010
536535
586535
59964
70964

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