| Column | Ultisil® ODS-3（4.6×250mm，5μm) |
| Mobile Phase | See the table below for details (0.01mol/L ammonium formate: Take 0.63g ammonium formate, add 1000mL Wahaha water to dissolve, adjust the pH to 3.0 with formic acid, shake well, and ultrasonic degassification to obtain. Acetonitrile: chromatographic grade acetonitrile is obtained.) |
| Detection | 254nm |
| Temperature | 35℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 10 μL |
Mobile Phase:
| Time (min) | 0.01 mol/L ammonium formate | Acetonitrile |
| 0 | 96 | 4 |
| 8 | 96 | 4 |
| 18 | 90 | 10 |
| 23 | 90 | 10 |
| 53 | 65 | 35 |
| 58 | 65 | 35 |
| 59 | 96 | 4 |
| 70 | 96 | 4 |
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