
Column | Ultisil® ODS-3(4.6×250mm,5μm) |
Mobile Phase | See the table below for details (0.01mol/L ammonium formate: Take 0.63g ammonium formate, add 1000mL Wahaha water to dissolve, adjust the pH to 3.0 with formic acid, shake well, and ultrasonic degassification to obtain. Acetonitrile: chromatographic grade acetonitrile is obtained.) |
Detection | 254nm |
Temperature | 35℃ |
Flow Rate | 1.0 ml/min |
Injection Volume | 10 μL |
Mobile Phase:
Time (min) | 0.01 mol/L ammonium formate | Acetonitrile |
0 | 96 | 4 |
8 | 96 | 4 |
18 | 90 | 10 |
23 | 90 | 10 |
53 | 65 | 35 |
58 | 65 | 35 |
59 | 96 | 4 |
70 | 96 | 4 |
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