Blank excipient solution
| Column | Ultisil® ODS-3（4.6×250mm，5μm) |
| Detection | 254nm |
| Temperature | 35℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 10ul |
| Note | / |
Mobile Phase
0.01mol/l ammonium formate: take 0.63g ammonium formate, dissolve it in 1000ml of water, and use a Adjust the pH of acid to 3.0, shake well and degass with ultrasonic. Acetonitrile: take chromatographic grade acetonitrile to obtain
| Time(min） | 0.01mol/l ammonium formate | Time (min): 0.01mol/l ammonium formate acetonitrile |
| 0 | 96 | 4 |
| 8 | 96 | 4 |
| 18 | 90 | 4 |
| 23 | 90 | 10 |
| 53 | 65 | 35 |
| 58 | 65 | 35 |
| 59 | 96 | 4 |
| 70 | 96 | 4 |
0 Comments