ColumnUltisil®Polar-RP(4.6×250mm,5μm)
Mobile PhasePhosphate buffer/acetonitrile=85/15
(weigh 4.09g of anhydrous dipotassium hydrogen phosphate and 0.58g of
potassium dihydrogen phosphate, dissolve in 1000ml of Wahaha water, mix well
and filter with suction)
Detection210nm
Temperature30℃
Flow Rate1.0 ml/min
Injection Volume20 μL

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