ColumnUltisil®Polar-RP(4.6×250mm,5μm)
Mobile PhasePhosphate buffer solution/acetonitrile=85/15 (weigh 4.09g of anhydrous
dipotassium hydrogen phosphate and 0.58g of potassium dihydrogen phosphate,
dissolve them in 1000ml of Wahaha water, mix well and filter with suction)
Detection210nm
Temperature30℃
Flow Rate1.0 ml/min
Injection Volume20 μL

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