
Column | Ultisil®Polar-RP(4.6×250mm,5μm) |
Mobile Phase | Phosphate buffer solution/acetonitrile=85/15 (weigh 4.09g of anhydrous dipotassium hydrogen phosphate and 0.58g of potassium dihydrogen phosphate, dissolve them in 1000ml of Wahaha water, mix well and filter with suction) |
Detection | 210nm |
Temperature | 30℃ |
Flow Rate | 1.0 ml/min |
Injection Volume | 20 μL |
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