| Column | Ultisil®Polar-RP（4.6×250mm，5μm) |
| Mobile Phase | Phosphate buffer solution/acetonitrile=85/15 (weigh 4.09g of anhydrous dipotassium hydrogen phosphate and 0.58g of potassium dihydrogen phosphate, dissolve them in 1000ml of Wahaha water, mix well and filter with suction) |
| Detection | 210nm |
| Temperature | 30℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 20 μL |
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