| Column | Ultisil® Polar-RP（4.6×250mm，5μm) |
| Mobile Phase | See the table below for details |
| Detection | 215nm |
| Temperature | 25℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 10 μL |
Mobile Phase:
Mobile phase A: 0.05mol/L potassium dihydrogen phosphate aqueous solution (adjust pH to 3.0 with phosphoric acid). Mobile Phase B: Acetonitrile
| Time (min) | Mobile phase A(%) | Mobile phase B(%) |
| 0 | 90 | 10 |
| 30 | 60 | 40 |
| 40 | 60 | 40 |
| 45 | 90 | 10 |
| 60 | 90 | 10 |
If you have any problem or require further information, please contact info@welchmat.com.
0 Comments